4/24/2023 0 Comments Tcr repertoire analysisOverview of major applications of TCR Repertoire profiling in a) Therapeutics application: TCR Sequencing of lymphocytes to identify antigen specific TCRs to generate engineered T cells for adoptive cell therapy b) Diagnostics assay for clonality for auto immune disorders c) Treatment monitoring: to identify biomarkers of response. Major Applications of TCR Repertoire Profiling Figure 1. To learn more about the different applications and sample data generated using TCR Seq at MedGenome read the white paper. At MedGenome, we provide TCR repertoire profiling using bulk input (from cells, RNA and FFPE tissue) using the SMARTer ® TCR Profiling Kit (Takara Bio USA Inc) and single-cell inputs using the SMARTer single-cell TCR Profiling kit and the Chromium Immune Profiling solutions (10X Genomics). Next-generation sequencing (NGS) technologies have recently enabled accurate detection of TCRs, and in combination with other assays allow for the assessment of the TCR repertoire in patients- which in turn is a proxy for patient prognosis and response. However identification of all potential clonotypes in a diverse repertoire of TCRs requires sensitive methods of detection. This study aimed to determine the characteristics and clinical significance of the TCR repertoire in lung cancer. Interestingly, the anti-CD4 mAb treatment-induced expansion of overlapping clones occurred mainly. TCR profiling holds great potential not only for understanding the mechanisms of development of the normal immune response, but also in providing insights into disease mechanisms and development of new therapeutics and treatment modalities in infectious diseases, autoimmunity and in immuno-oncology. Sequencing of the T-cell receptor (TCR) repertoire can reveal the immunosurveillance status of the tumor microenvironment, which is related to tumor escape and immunotherapy. By Inter-Organ Clone Tracking analysis, we demonstrated that anti-CD4 mAb treatment increased the diversity and combined frequency of CD8 + T cell clones that overlapped among the tumor, draining lymph node (dLN), and peripheral blood repertoires. Productive T-cell activation results in the clonal expansion of a specific T-cell and this expansion can be accurately determined by TCR sequencing. Each TCR recognize peptides in complex with MHC proteins presented on the surface of antigen presenting cells. This highly diverse repertoire of T-cells has the ability to recognize peptides originating from foreign elements such as invading pathogens and cancer cells. Humans carry >10 9 T-cells, each expressing a unique TCR. The activation of T-cells involve recognition of MHC-peptide complex by the T-cell receptors (TCR). Once activated, they can directly kill cells that are foreign (cytolytic T-cells) or perform helper function (helper T-cells) to activate B-cells to make antibodies against foreign antigen. T-cells are the core components of our adaptive immune system.
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